What’s the Difference between UPLC and HPLC?

UPLC and HPLC are both used to separate components found in mixtures. However, the similarities in these liquid chromatography techniques end there, as UPLC (Ultra Performance Liquid Chromatography) operates at higher pressures (15,000 psi) and allows for lower particle sizes in columns, while HPLC (High Performance Liquid Chromatography) operates at lower pressures (max 500-6000 psi). This presents an advantage over HPLC in improved analyte separation and detection, lower solvent consumption, and shorter run times.

Ferndale Contract Manufacturing has over 30 years of experience employing HPLC techniques and has started utilizing UPLC systems over the last four years. The main advantages of UPLC over HPLC are rapid turnaround times for analytical testing, and enhanced separations and optimal product profile on an analytical scale. HPLC is still available, used when transferring or developing and validating an existing compendial method that already uses HPLC.

Ferndale Contract Manufacturing’s QC analysts have over 100+ combined years of analytical chromatographic experience. This experience guides the development and implementation of methodology that is the best fit for a given application.

If you’d like to learn more about UPLC and HPLC, or any of the other services Ferndale Contract Manufacturing provides, please call 877-548-0122 or fill out a contact form on our website.